RasGAP IB1 LDL

The general aim of our laboratory is to understand the role of signaling proteins in apoptosis and other cellular responses. The signaling proteins we focus on are involved in the regulation of mitogen activated protein kinase (MAPK) pathways. Three main avenues of investigations are currently being pursued in the laboratory:

1) Role of the cleavage of RasGAP by caspases during apoptosis.
2) Regulation of MAPK expression levels by scaffold proteins.
3) Characterization of the intracellular signals induced by lipoproteins.

More information about these projects can be accessed through the following link

RasGAP project	IB1 project	LDLs project

The MAPK pathways

Recent news

Mol Biol Cell. 2005 Aug;16(8):3511-20	Mol Cell Biol. 2004 Dec;24(23):10425-36.

Impaired Akt Activity Down-Modulation, Caspase-3 Activation, and Apoptosis in Cells Expressing a Caspase-resistant Mutant of RasGAP at Position 157. Cells expressing the D157A mutant of RasGAP display selective survival capacity. Wild-type RasGAP-, D455A-, or D157A-expressing MEFs were stained with blue, green, and orange dyes, respectively, as indicated in Materials and Methods. The cells were then mixed in equal proportion and incubated with the indicated cisplatin concentrations. The proportion of the different colored cells remaining alive after 24 or 48 h was then assessed.	Partial Cleavage of RasGAP by Caspases Is Required for Cell Survival in Mild Stress Conditions RasGAP–/– cells stably expressing plasmids encoding either wild-type RasGAP (second row), or the uncleavable D455A mutant (two independent clones; bottom rows) were plated in 10-cm dishes and incubated for 8 days in the absence (left lane) or in the presence (right lane) of 0.5 µM cisplatin.

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last update: 31.01.2006